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Phosphatidylinositol 4,5-Bisphosphate Increases Ca(2+) Affinity of Synaptotagmin-1 by 40-fold
Synaptotagmin-1 is the main Ca(2+) sensor of neuronal exocytosis. It binds to both Ca(2+) and the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)), but the precise cooperativity of this binding is still poorly understood. Here, we used microscale thermophoresis to quantify the coo...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3351315/ https://ncbi.nlm.nih.gov/pubmed/22447935 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.343418 |
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