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RNase H cleavage of RNA hybridized to oligonucleotides containing methylphosphonate, phosphorothioate and phosphodiester bonds.

Three types of 14-mer oligonucleotides were hybridized to human beta-globin pre-mRNA and the resultant duplexes were tested for susceptibility to cleavage by RNase H from E. coli or from HeLa cell nuclear extract. The oligonucleotides contained normal deoxynucleotides, phosphorothioate analogs alter...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Furdon, P J, Dominski, Z, Kole, R
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 1989
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335124/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2555787/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.22.9193
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