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RNase H cleavage of RNA hybridized to oligonucleotides containing methylphosphonate, phosphorothioate and phosphodiester bonds.

Three types of 14-mer oligonucleotides were hybridized to human beta-globin pre-mRNA and the resultant duplexes were tested for susceptibility to cleavage by RNase H from E. coli or from HeLa cell nuclear extract. The oligonucleotides contained normal deoxynucleotides, phosphorothioate analogs alter...

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Bibliografske podrobnosti
Main Authors: Furdon, P J, Dominski, Z, Kole, R
Format: Artigo
Jezik:Inglês
Izdano: 1989
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC335124/
https://ncbi.nlm.nih.gov/pubmed/2555787
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