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RNase H cleavage of RNA hybridized to oligonucleotides containing methylphosphonate, phosphorothioate and phosphodiester bonds.
Three types of 14-mer oligonucleotides were hybridized to human beta-globin pre-mRNA and the resultant duplexes were tested for susceptibility to cleavage by RNase H from E. coli or from HeLa cell nuclear extract. The oligonucleotides contained normal deoxynucleotides, phosphorothioate analogs alter...
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| Опубликовано в: : | Nucleic Acids Res |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
1989
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| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335124/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2555787/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.22.9193 |
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