PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.

A novel alternative to microcloning for the production of region specific chromosomal DNA is described. In this method, 'microamplification', single bands are dissected from polytene chromosomes and digested with Sau3A. Oligonucleotide adaptors are ligated to these fragments to provide con...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Saunders, R D, Glover, D M, Ashburner, M, Siden-Kiamos, I, Louis, C, Monastirioti, M, Savakis, C, Kafatos, F
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1989
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335111/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2587252/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.22.9027
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