PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.

A novel alternative to microcloning for the production of region specific chromosomal DNA is described. In this method, 'microamplification', single bands are dissected from polytene chromosomes and digested with Sau3A. Oligonucleotide adaptors are ligated to these fragments to provide convenient pr...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Saunders, R D, Glover, D M, Ashburner, M, Siden-Kiamos, I, Louis, C, Monastirioti, M, Savakis, C, Kafatos, F
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1989
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335111/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2587252/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.22.9027
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