PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.

A novel alternative to microcloning for the production of region specific chromosomal DNA is described. In this method, 'microamplification', single bands are dissected from polytene chromosomes and digested with Sau3A. Oligonucleotide adaptors are ligated to these fragments to provide con...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Saunders, R D, Glover, D M, Ashburner, M, Siden-Kiamos, I, Louis, C, Monastirioti, M, Savakis, C, Kafatos, F
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1989
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335111/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2587252/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.22.9027
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