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Cloning regions of the Drosophila genome by microdissection of polytene chromosome DNA and PCR with nonspecific primer.
A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspeci...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1990
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC333467/ https://ncbi.nlm.nih.gov/pubmed/2106670 |
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