Isolation of region-specific cosmids from chromosome 5 by hybridization with microdissection clones.

A method is described for the isolation of chromosome region specific cosmids. The 5q35 region of the long arm of human chromosome 5 was microdissected, digested with MboI, ligated to oligonucleotide adaptors, amplified by the polymerase chain reaction and cloned into a plasmid vector. Inserts which...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nucleic Acids Res
Egile Nagusiak: Saltman, D L, Dolganov, G M, Pearce, B S, Kuo, S S, Callahan, P J, Cleary, M L, Lovett, M
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 1992
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312189/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1561096/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.6.1401
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