Isolation of region-specific cosmids from chromosome 5 by hybridization with microdissection clones.

A method is described for the isolation of chromosome region specific cosmids. The 5q35 region of the long arm of human chromosome 5 was microdissected, digested with MboI, ligated to oligonucleotide adaptors, amplified by the polymerase chain reaction and cloned into a plasmid vector. Inserts which...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Saltman, D L, Dolganov, G M, Pearce, B S, Kuo, S S, Callahan, P J, Cleary, M L, Lovett, M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1992
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312189/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1561096/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.6.1401
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