Isolation of region-specific cosmids from chromosome 5 by hybridization with microdissection clones.
A method is described for the isolation of chromosome region specific cosmids. The 5q35 region of the long arm of human chromosome 5 was microdissected, digested with MboI, ligated to oligonucleotide adaptors, amplified by the polymerase chain reaction and cloned into a plasmid vector. Inserts which...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1992
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312189/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1561096/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.6.1401 |
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