Overproduction and crystallization of FokI restriction endonuclease.

To overproduce FokI endonuclease (R.FokI) in an Escherichia coli system, the coding region of R.FokI predicted from the nucleotide sequence was generated from the FokI operon and joined to the tac promoter of an expression vector, pKK223-3. By introduction of the plasmid into E. coli UT481 cells exp...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Kita, K, Kotani, H, Hiraoka, N, Nakamura, T, Yonaha, K
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1989
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335040/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2685747/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.21.8741
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