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Overproduction and crystallization of FokI restriction endonuclease.

To overproduce FokI endonuclease (R.FokI) in an Escherichia coli system, the coding region of R.FokI predicted from the nucleotide sequence was generated from the FokI operon and joined to the tac promoter of an expression vector, pKK223-3. By introduction of the plasmid into E. coli UT481 cells exp...

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Detaylı Bibliyografya
Yayımlandı:Nucleic Acids Res
Asıl Yazarlar: Kita, K, Kotani, H, Hiraoka, N, Nakamura, T, Yonaha, K
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 1989
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335040/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2685747/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.21.8741
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