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Overproduction and crystallization of FokI restriction endonuclease.

To overproduce FokI endonuclease (R.FokI) in an Escherichia coli system, the coding region of R.FokI predicted from the nucleotide sequence was generated from the FokI operon and joined to the tac promoter of an expression vector, pKK223-3. By introduction of the plasmid into E. coli UT481 cells exp...

詳細記述

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書誌詳細
出版年:Nucleic Acids Res
主要な著者: Kita, K, Kotani, H, Hiraoka, N, Nakamura, T, Yonaha, K
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1989
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335040/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2685747/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.21.8741
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