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Catalysis by a de novo zinc-mediated protein interface: implications for natural enzyme evolution and rational enzyme engineering

Here we show that a recent computationally designed zinc-mediated protein interface is serendipitously capable of catalyzing carboxyester and phosphoester hydrolysis. Although the original motivation was to design a de novo zinc-mediated protein-protein interaction (called MID1-zinc), we observed in...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Der, Bryan S., Edwards, David R., Kuhlman, Brian
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3348550/
https://ncbi.nlm.nih.gov/pubmed/22510088
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201881p
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