Nucleotide Pocket Thermodynamics Measured by EPR Reveal How Energy Partitioning Relates Speed to Efficiency

We have used spin-labeled ADP to investigate the dynamics of the nucleotide-binding pocket in a series of myosins, which have a range of velocities. Electron Paramagnetic Resonance spectroscopy reveals that the pocket is in an equilibrium between open and closed conformations. In the absence of acti...

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Bibliografiske detaljer
Main Authors: Purcell, Thomas J., Naber, Nariman, Franks-Skiba, Kathy, Dunn, Alexander R., Eldred, Catherine C., Berger, Christopher L., Málnási-Csizmadia, András, Spudich, James A., Swank, Douglas M., Pate, Edward, Cooke, Roger
Format: Artigo
Sprog:Inglês
Udgivet: 2010
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3347976/
https://ncbi.nlm.nih.gov/pubmed/21185304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.11.053
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