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Dynamics of the Nucleotide Pocket of Myosin Measured by Spin-Labeled Nucleotides

We have used electron paramagnetic probes attached to the ribose of ATP (SL-ATP) to monitor conformational changes in the nucleotide pocket of myosin. Spectra for analogs bound to myosin in the absence of actin showed a high degree of immobilization, indicating a closed nucleotide pocket. In the Act...

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Autori principali: Naber, Nariman, Purcell, Thomas J., Pate, Edward, Cooke, Roger
Natura: Artigo
Lingua:Inglês
Pubblicazione: Biophysical Society 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1697850/
https://ncbi.nlm.nih.gov/pubmed/17028139
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.090035
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