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Preparation of 13C and 15N labelled RNAs for heteronuclear multi-dimensional NMR studies.
A procedure is described for the efficient preparation of isotopically enriched RNAs of defined sequence. Uniformly labelled nucleotide 5'triphosphates (NTPs) were prepared from E.coli grown on 13C and/or 15N isotopically enriched media. These procedures routinely yield 180 mumoles of labelled...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1992
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| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334178/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1383927/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.17.4507 |
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