Preparation of 13C and 15N labelled RNAs for heteronuclear multi-dimensional NMR studies.

A procedure is described for the efficient preparation of isotopically enriched RNAs of defined sequence. Uniformly labelled nucleotide 5'triphosphates (NTPs) were prepared from E.coli grown on 13C and/or 15N isotopically enriched media. These procedures routinely yield 180 mumoles of labelled...

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Publicat a:Nucleic Acids Res
Autors principals: Nikonowicz, E P, Sirr, A, Legault, P, Jucker, F M, Baer, L M, Pardi, A
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1992
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334178/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1383927/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.17.4507
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