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A continuous fluorescent enzyme assay for early steps of lipid A biosynthesis

UDP-N-acetylglucosamine acyltransferase (LpxA) and UDP-3-O-(R-3-hydroxyacyl)-glucosamine acyltransferase (LpxD) catalyze the first and third steps of Lipid A biosynthesis, respectively. Both enzymes have been found to be essential for survival among Gram-negative bacteria which synthesize lipopolysa...

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Autori principali: Jenkins, Ronald J., Dotson, Garry D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3338867/
https://ncbi.nlm.nih.gov/pubmed/22381368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.02.027
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