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Sensitive quantitative analysis of murine LINE1 DNA methylation using high resolution melt analysis

We present here the first high resolution melt (HRM) assay to quantitatively analyze differences in murine DNA methylation levels utilizing CpG methylation of Long Interspersed Elements-1 (LINE1 or L1). By calculating the integral difference in melt temperature between samples and a methylated contr...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Newman, Michelle, Blyth, Benjamin J., Hussey, Damian J., Jardine, Daniel, Sykes, Pamela J., Ormsby, Rebecca J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Landes Bioscience 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3337834/
https://ncbi.nlm.nih.gov/pubmed/22274612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/epi.7.1.18815
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