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Introduction of a Disulfide Bond Leads to Stabilization and Crystallization of a Ricin Immunogen()

RTA1-33/44-198 is a catalytically inactive, single-domain derivative of the ricin toxin A-chain (RTA) engineered to serve as a stable protein scaffold for presentation of native immunogenic epitopes (Olson, et al., Protein Eng Des Sel 2004 17:391-7). To improve the stability and solubility of RTA1-3...

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Main Authors: Compton, Jaimee R., Legler, Patricia M., Clingan, Benjamin V., Olson, Mark A., Millard, Charles B.
格式: Artigo
語言:Inglês
出版: 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3332088/
https://ncbi.nlm.nih.gov/pubmed/21387408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prot.22933
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