Preparation and characterization of yeast nuclear extracts for efficient RNA polymerase B (II)-dependent transcription in vitro.

We present a reproducible method for the preparation of nuclear extracts from the yeast Saccharomyces cerevisiae that support efficient RNA polymerase B (II)-dependent transcription. Extracts from both a crude nuclear fraction and Percoll-purified nuclei are highly active for site-specific initiatio...

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Autores principales: Verdier, J M, Stalder, R, Roberge, M, Amati, B, Sentenac, A, Gasser, S M
Formato: Artigo
Lenguaje:Inglês
Publicado: 1990
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC332766/
https://ncbi.nlm.nih.gov/pubmed/2263463
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