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Preparation and characterization of yeast nuclear extracts for efficient RNA polymerase B (II)-dependent transcription in vitro.
We present a reproducible method for the preparation of nuclear extracts from the yeast Saccharomyces cerevisiae that support efficient RNA polymerase B (II)-dependent transcription. Extracts from both a crude nuclear fraction and Percoll-purified nuclei are highly active for site-specific initiatio...
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| Main Authors: | , , , , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1990
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| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC332766/ https://ncbi.nlm.nih.gov/pubmed/2263463 |
| Oznake: |
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