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Coupled RNA polymerase II transcription and 3′ end formation with yeast whole-cell extracts
RNA polymerase II (RNAP II) transcription and pre-mRNA 3′ end formation are linked through physical and functional interactions. We describe here a highly efficient yeast in vitro system that reproduces both transcription and 3′ end formation in a single reaction. The system is based on simple whole...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Cold Spring Harbor Laboratory Press
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2957059/ https://ncbi.nlm.nih.gov/pubmed/20810619 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2172510 |
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