Coupled RNA polymerase II transcription and 3′ end formation with yeast whole-cell extracts

RNA polymerase II (RNAP II) transcription and pre-mRNA 3′ end formation are linked through physical and functional interactions. We describe here a highly efficient yeast in vitro system that reproduces both transcription and 3′ end formation in a single reaction. The system is based on simple whole...

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Bibliographic Details
Main Authors: Mariconti, Luisa, Loll, Bernhard, Schlinkmann, Karola, Wengi, Agnieszka, Meinhart, Anton, Dichtl, Bernhard
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2010
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957059/
https://ncbi.nlm.nih.gov/pubmed/20810619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2172510
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