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Isolation and characterization of a variant dihydrofolate reductase cDNA from methotrexate-resistant murine L5178Y cells.

Dihydrofolate reductase (DHFR) cDNA sequences were isolated from a methotrexate-resistant mouse L5178Y cell line previously shown to contain methotrexate-resistant dihydrofolate reductase enzyme activity. Specifically-primed reverse transcription products were amplified using the polymerase chain re...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: McIvor, R S, Simonsen, C C
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1990
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332765/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2263462/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.23.7025
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