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UV-induced cross-linking of Tet repressor to DNA containing tet operator sequences and 8-azidoadenines.

The synthesis of 8-azido-2'-deoxyadenosine-5'-triphosphate is described. The photoreactive dATP analog was characterized by thin layer chromatography, proton resonance spectroscopy, infrared spectroscopy and UV spectroscopy. Its photolysis upon UV irradiation was studied. After incorporation of this...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Meffert, R, Rathgeber, G, Schäfer, H J, Dose, K
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1990
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332621/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2251123/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.22.6633
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