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UV-induced cross-linking of Tet repressor to DNA containing tet operator sequences and 8-azidoadenines.

The synthesis of 8-azido-2'-deoxyadenosine-5'-triphosphate is described. The photoreactive dATP analog was characterized by thin layer chromatography, proton resonance spectroscopy, infrared spectroscopy and UV spectroscopy. Its photolysis upon UV irradiation was studied. After incorporation of this...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Principais autores: Meffert, R, Rathgeber, G, Schäfer, H J, Dose, K
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1990
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332621/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2251123/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.22.6633
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