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UV-induced cross-linking of Tet repressor to DNA containing tet operator sequences and 8-azidoadenines.

The synthesis of 8-azido-2'-deoxyadenosine-5'-triphosphate is described. The photoreactive dATP analog was characterized by thin layer chromatography, proton resonance spectroscopy, infrared spectroscopy and UV spectroscopy. Its photolysis upon UV irradiation was studied. After incorporation of this...

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Publicat a:Nucleic Acids Res
Autors principals: Meffert, R, Rathgeber, G, Schäfer, H J, Dose, K
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1990
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332621/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2251123/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.22.6633
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