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UV-induced cross-linking of Tet repressor to DNA containing tet operator sequences and 8-azidoadenines.

The synthesis of 8-azido-2'-deoxyadenosine-5'-triphosphate is described. The photoreactive dATP analog was characterized by thin layer chromatography, proton resonance spectroscopy, infrared spectroscopy and UV spectroscopy. Its photolysis upon UV irradiation was studied. After incorporation of this...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Meffert, R, Rathgeber, G, Schäfer, H J, Dose, K
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 1990
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332621/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2251123/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.22.6633
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