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Single Molecule FRET of Protein-Nucleic Acid and Protein-Protein complexes: Surface Passivation and Immobilization

Single-molecule fluorescence spectroscopy reveals the real time dynamics that occur during biomolecular interactions that would otherwise be hidden by the ensemble average. It also removes the requirement to synchronize reactions, thus providing a very intuitive approach to study kinetics of biologi...

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Autores principales: Lamichhane, Rajan, Solem, Amanda, Black, Will, Rueda, David
Formato: Artigo
Lenguaje:Inglês
Publicado: 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3321382/
https://ncbi.nlm.nih.gov/pubmed/20554047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.06.010
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