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Single Molecule FRET of Protein-Nucleic Acid and Protein-Protein complexes: Surface Passivation and Immobilization

Single-molecule fluorescence spectroscopy reveals the real time dynamics that occur during biomolecular interactions that would otherwise be hidden by the ensemble average. It also removes the requirement to synchronize reactions, thus providing a very intuitive approach to study kinetics of biologi...

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Detalhes bibliográficos
Main Authors: Lamichhane, Rajan, Solem, Amanda, Black, Will, Rueda, David
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3321382/
https://ncbi.nlm.nih.gov/pubmed/20554047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.06.010
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