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Amplified inverted duplications within and adjacent to heterologous selectable DNA.

Plasmids containing a dihydrofolate reductase (DHFR) expression unit were transfected into DHFR-deficient Chinese hamster ovary (CHO) cells. Methotrexate exposure was used to select cells with amplified DHFR sequences. Three cell lines were isolated containing amplified copies of transfected DNA tha...

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Bibliografski detalji
Izdano u:Nucleic Acids Res
Glavni autori: Heartlein, M W, Latt, S A
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1989
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331829/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2922290/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.4.1697
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