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Amplified inverted duplications within and adjacent to heterologous selectable DNA.
Plasmids containing a dihydrofolate reductase (DHFR) expression unit were transfected into DHFR-deficient Chinese hamster ovary (CHO) cells. Methotrexate exposure was used to select cells with amplified DHFR sequences. Three cell lines were isolated containing amplified copies of transfected DNA tha...
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| Udgivet i: | Nucleic Acids Res |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1989
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331829/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2922290/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.4.1697 |
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