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Amplified inverted duplications within and adjacent to heterologous selectable DNA.
Plasmids containing a dihydrofolate reductase (DHFR) expression unit were transfected into DHFR-deficient Chinese hamster ovary (CHO) cells. Methotrexate exposure was used to select cells with amplified DHFR sequences. Three cell lines were isolated containing amplified copies of transfected DNA tha...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1989
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC331829/ https://ncbi.nlm.nih.gov/pubmed/2922290 |
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