Nuclear translation visualized by ribosome-bound nascent chain puromycylation

Whether protein translation occurs in the nucleus is contentious. To address this question, we developed the ribopuromycylation method (RPM), which visualizes translation in cells via standard immunofluorescence microscopy. The RPM is based on ribosome-catalyzed puromycylation of nascent chains immo...

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Hlavní autoři: David, Alexandre, Dolan, Brian P., Hickman, Heather D., Knowlton, Jonathan J., Clavarino, Giovanna, Pierre, Philippe, Bennink, Jack R., Yewdell, Jonathan W.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Rockefeller University Press 2012
Témata:
Research Articles
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3317795/
https://ncbi.nlm.nih.gov/pubmed/22472439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201112145
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