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Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes

Puromycin is an amino-acyl transfer RNA analog widely employed in studies of protein synthesis. Since puromycin is covalently incorporated into nascent polypeptide chains, anti-puromycin immunofluorescence enables visualization of nascent protein synthesis. A common assumption in studies of local me...

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Bibliographic Details
Published in:eLife
Main Authors: Hobson, Benjamin D, Kong, Linghao, Hartwick, Erik W, Gonzalez, Ruben L, Sims, Peter A
Format: Artigo
Language:Inglês
Published: eLife Sciences Publications, Ltd 2020
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7490010/
https://ncbi.nlm.nih.gov/pubmed/32844746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.60048
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