Nuclear translation visualized by ribosome-bound nascent chain puromycylation

Whether protein translation occurs in the nucleus is contentious. To address this question, we developed the ribopuromycylation method (RPM), which visualizes translation in cells via standard immunofluorescence microscopy. The RPM is based on ribosome-catalyzed puromycylation of nascent chains immo...

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Bibliographic Details
Main Authors: David, Alexandre, Dolan, Brian P., Hickman, Heather D., Knowlton, Jonathan J., Clavarino, Giovanna, Pierre, Philippe, Bennink, Jack R., Yewdell, Jonathan W.
Format: Artigo
Language:Inglês
Published: The Rockefeller University Press 2012
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Research Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3317795/
https://ncbi.nlm.nih.gov/pubmed/22472439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201112145
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