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Nuclear translation visualized by ribosome-bound nascent chain puromycylation
Whether protein translation occurs in the nucleus is contentious. To address this question, we developed the ribopuromycylation method (RPM), which visualizes translation in cells via standard immunofluorescence microscopy. The RPM is based on ribosome-catalyzed puromycylation of nascent chains immo...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
The Rockefeller University Press
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3317795/ https://ncbi.nlm.nih.gov/pubmed/22472439 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201112145 |
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