Skip the Alignment: Degenerate, Multiplex Primer and Probe Design Using K-mer Matching Instead of Alignments

PriMux is a new software package for selecting multiplex compatible, degenerate primers and probes to detect diverse targets such as viruses. It requires no multiple sequence alignment, instead applying k-mer algorithms, hence it scales well for large target sets and saves user effort from curating...

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Bibliografiske detaljer
Main Authors: Hysom, David A., Naraghi-Arani, Pejman, Elsheikh, Maher, Carrillo, A. Celena, Williams, Peter L., Gardner, Shea N.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2012
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3317645/
https://ncbi.nlm.nih.gov/pubmed/22485178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0034560
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