An essential amino acid residue in the protein translocation channel revealed by targeted random mutagenesis of SecY
The SecY/Sec61α family of membrane proteins are the central subunits of the putative protein translocation channel. We introduced random mutations into a segment of Escherichia coli SecY within its cytoplasmic domain 5, which was shown previously to be important for the SecA-dependent translocation...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC33175/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11309495/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.081617398 |
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