Library subtraction of in vitro cDNA libraries to identify differentially expressed genes in scrapie infection.
We have developed a system where double-stranded cDNA can be amplified using a synthetic oligonucleotide primer and the polymerase chain reaction, generating cDNA libraries in vitro. Using a library subtraction strategy (1), scrapie and control brain in vitro cDNA libraries were used to identify seq...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1990
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330765/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2339063/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.9.2789 |
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