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Library subtraction of in vitro cDNA libraries to identify differentially expressed genes in scrapie infection.

We have developed a system where double-stranded cDNA can be amplified using a synthetic oligonucleotide primer and the polymerase chain reaction, generating cDNA libraries in vitro. Using a library subtraction strategy (1), scrapie and control brain in vitro cDNA libraries were used to identify seq...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Duguid, J R, Dinauer, M C
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1990
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330765/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2339063/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.9.2789
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