Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes

In the effort to prepare the mouse full-length cDNA encyclopedia, we previously developed several techniques to prepare and select full-length cDNAs. To increase the number of different cDNAs, we introduce here a strategy to prepare normalized and subtracted cDNA libraries in a single step. The meth...

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Autori principali: Carninci, Piero, Shibata, Yuko, Hayatsu, Norihito, Sugahara, Yuichi, Shibata, Kazuhiro, Itoh, Masayoshi, Konno, Hideaki, Okazaki, Yasushi, Muramatsu, Masami, Hayashizaki, Yoshihide
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2000
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC310980/
https://ncbi.nlm.nih.gov/pubmed/11042159
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