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Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes

In the effort to prepare the mouse full-length cDNA encyclopedia, we previously developed several techniques to prepare and select full-length cDNAs. To increase the number of different cDNAs, we introduce here a strategy to prepare normalized and subtracted cDNA libraries in a single step. The meth...

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Udgivet i:Genome Res
Principais autores: Carninci, Piero, Shibata, Yuko, Hayatsu, Norihito, Sugahara, Yuichi, Shibata, Kazuhiro, Itoh, Masayoshi, Konno, Hideaki, Okazaki, Yasushi, Muramatsu, Masami, Hayashizaki, Yoshihide
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2000
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Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310980/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11042159/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.145100
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