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Library subtraction of in vitro cDNA libraries to identify differentially expressed genes in scrapie infection.
We have developed a system where double-stranded cDNA can be amplified using a synthetic oligonucleotide primer and the polymerase chain reaction, generating cDNA libraries in vitro. Using a library subtraction strategy (1), scrapie and control brain in vitro cDNA libraries were used to identify seq...
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| Huvudupphovsmän: | , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1990
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC330765/ https://ncbi.nlm.nih.gov/pubmed/2339063 |
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