Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments.

We have developed a fast and general method to obtain an enriched, full-length cDNA expression library with subtractively enriched cDNA fragments. The procedure relies on RecA-mediated triple-helix formation of single-stranded cDNA fragments with a double-stranded cDNA plasmid library. The complexes...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Hakvoort, T B, Spijkers, J A, Vermeulen, J L, Lamers, W H
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1996
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146116/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8811109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.17.3478
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