Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments.

We have developed a fast and general method to obtain an enriched, full-length cDNA expression library with subtractively enriched cDNA fragments. The procedure relies on RecA-mediated triple-helix formation of single-stranded cDNA fragments with a double-stranded cDNA plasmid library. The complexes...

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Main Authors: Hakvoort, T B, Spijkers, J A, Vermeulen, J L, Lamers, W H
Format: Artigo
Jezik:Inglês
Izdano: 1996
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC146116/
https://ncbi.nlm.nih.gov/pubmed/8811109
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