Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments.

We have developed a fast and general method to obtain an enriched, full-length cDNA expression library with subtractively enriched cDNA fragments. The procedure relies on RecA-mediated triple-helix formation of single-stranded cDNA fragments with a double-stranded cDNA plasmid library. The complexes...

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書誌詳細
出版年:	Nucleic Acids Res
主要な著者:	Hakvoort, T B, Spijkers, J A, Vermeulen, J L, Lamers, W H
フォーマット:	Artigo
言語:	Inglês
出版事項:	Oxford University Press 1996
主題:	Research Article
オンライン･アクセス:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146116/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8811109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.17.3478
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Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments.

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