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High-Stringency Subtraction for the Identification of Differentially Regulated cDNA Clones

The technique of high-stringency subtraction described here facilitates subtractive hybridizations between directional cDNA libraries constructed in λ ZAP® II cloning vectors and represents an improvement on earlier methods for the subtraction of entire cDNA libraries. High-stringency subtraction is...

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Bibliografiske detaljer
Principais autores: Charles P. Scutt, Philip M. Gilmartin
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis Group 1997-09-01
Serier:BioTechniques
Online adgang:https://www.future-science.com/doi/10.2144/97233st04
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