A method for generating subtractive cDNA libraries retaining clones containing repetitive elements.

Here we describe a two-stepped photobiotin-based procedure to enrich a target (canine retinal) cDNA library for tissue specific clones without removing those containing repetitive ( SINE ) elements, despite the presence of these elements in the driver population. In a first hybridization excess SINE...

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Autors principals: Lin, C T, Sargan, D R
Format: Artigo
Idioma:Inglês
Publicat: 1997
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC147059/
https://ncbi.nlm.nih.gov/pubmed/9336480
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