A method for generating subtractive cDNA libraries retaining clones containing repetitive elements.

Here we describe a two-stepped photobiotin-based procedure to enrich a target (canine retinal) cDNA library for tissue specific clones without removing those containing repetitive ( SINE ) elements, despite the presence of these elements in the driver population. In a first hybridization excess SINE...

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Bibliografski detalji

Izdano u:	Nucleic Acids Res
Glavni autori:	Lin, C T, Sargan, D R
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 1997
Teme:	Research Article
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147059/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9336480/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.21.4427
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