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A method for generating subtractive cDNA libraries retaining clones containing repetitive elements.

Here we describe a two-stepped photobiotin-based procedure to enrich a target (canine retinal) cDNA library for tissue specific clones without removing those containing repetitive ( SINE ) elements, despite the presence of these elements in the driver population. In a first hybridization excess SINE...

詳細記述

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書誌詳細
出版年:Nucleic Acids Res
主要な著者: Lin, C T, Sargan, D R
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147059/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9336480/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.21.4427
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