Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.
We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brai...
Saved in:
| Published in: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1988
|
| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC281836/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2456582/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.15.5738 |
| Tags: |
No Tags, Be the first to tag this record!
|